Urine Specimen and Culture 4.1. %%EOF nurses often assume the responsibility of specimen collection. Looks like youve clipped this slide to already. An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. $.' hb```WB cbC May perform venipunctures, including blood culture collection, and fingersticks ensuring proper /labeling of specimens and using vein preservation techniques. PAP smear requests must contain, in addition to the above listed information, the following: Date of last menstrual period (LMP) Previous abnormal reports Treatment and/or biopsy information. Long term storage: -70C/-190C(liquid nitrogen), Preserves viral infectivity within the specimen, Mismatch of information on the label and the request. Conversely, moving from upright to supine can have a dilutional effect owing to an increase in plasma volume. Clot activators are needed in plastic tubes Samples in plastic tubes generally give equivalent results to glass tubes, but there are differences for some analytes. Indicating the test(s) requested. If the specimen is irreplaceable the ordering physician must attest to the correct patients identity and must authorize (on the specimen relabeling form) affixation of the correct label by an attendance nurse. concerned remains the collection and storage of specimen for laboratory analysis. Tourniquet - Wipe off with alcohol and replace frequently. specimen #2. specimen #3. specimen #4. specimen #5. specimen #6. specimen # 7. learning objectives. Collect the specimen during the early phase of the disease and as far as possible before the administration of antimicrobial agents. We've updated our privacy policy. - Collection of Epidemiologic Data Bao-Ping Zhu, MD, MS - Title: Slide 1 Created Date: 11/20/2008 5:55:36 PM Document presentation format: On-screen Show (4:3) Other titles: Arial Majalla UI Calibri Traditional Arabic How Warring Medical Kits Provides Valued Protection for Specimen Collection. Viruses which can be cultured from blood include dengue viruses and some arboviruses. Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. microbiology. Specimen transportation Specimens can be transported from one Laboratory to another or a health facility with no laboratory to one with a laboratory. Closed system collection - collect from the catheter tubing. Two serum specimens (paired sera) are necessary to detect a rise in the titer of the Ab. Send to the virology laboratory in a cold box. . The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. collected in 10% formal saline (collection to be done in wide mouth bottles with 10 times the volume of tissues). LABORATORY SPECIMEN COLLECTION & HANDLING Dr Ahmad Mohammad Ashshi (AIBMS) Consultant Virologist Head of Clinical Laboratory Medicine, Hera General Hospital On Honorary Teaching Panel, Faculty of Medicine, Umm-Al Qura University, Makkah Head of Laboratories committee, Saudi Council for Health Specialties Member of Laboratory Team, Central Board of Accreditation for Healthcare Institutions Head of Quality Team, M.O.H, Makkah. Activate your 30 day free trialto unlock unlimited reading. PowerShow.com is a leading presentation sharing website. Recently purchased by the Land Trust for Santa Barbara County, the property is known for its significant natural, historical, and scenic values. Sputum Specimen and Culture 3. endstream endobj 367 0 obj <>stream What is the collection order of tubes? Follow patient instructions prior to specimen collection Including the proper order of blood draw when multiple tubes are required. Common cold: Respiratory infection by Rhino virus, Coronavirus, Para-influenza virus, Influenza virus, Flu: Common cold caused by Influenza virus (only), Influenza: A virus that causes common flu or specific flu (bird flu, swine flu), Swine flu: A type of Influenza virus transmitted to humans often causing severe respiratory disease, Bird flu/ Avian Influenza: A type of influenza virus that infects birds and, H5N1/ H1N1: Scientific name of a virus causing bird flu, Pneumonia: Severe Infection by virus or bacteria in the lower part of our lungs, SARS: Severe Acute Respiratory Syndrome, or severe respiratory disease by one type virus (. Appropriate shipping of specimen to the laboratory for testing. Proper sample collection leads to the proper diagnosis of disease. And, again, its all free. f47ztf9!oKBt-~Um93V3)7u;ZF-#H1M Completing each step correctly improves the likelihood the specimen can be successfully tested. Insertion of an in and out catheter just to collect the specimen. Regulations for transporting samples come from . All blood, whether unlabelled or mislabeled, intended for cross matching, must be redrawn, without exception. Most cellular and macromolecular analytes are 5 to 15% higher when specimens are collected erect as compared to supine. display false neg. Laboratory specimen: collection, safe transport and biosafety. More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. Processing of urine in microbiology by Rahul raj, Cytopathology Lab manual for MLT Students, Abnormalities of the Pupil copy copy copy.pptx, Westside Wellness - Mobile IV Hydration Therapy, Intensification Options after basal Insulin Revisited, health related behaviour ( ).pptx, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. a. specimen collection and transport. 2022. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. dr.t.v.rao md. Deliver specimens to the laboratory promptly Valid measurement of analytes in serum or plasma requires prompt separation from the blood cells. Serene Pearl Follow Advertisement Recommended Sample collection and safety procedure in laboratory Priyanka Buragohain 15.9k views 54 slides EDTA (Anti-Coagulant K2EDTA is used to collect whole blood for hematology studies and plasma for analytes with heparin interference Acts by binding calcium Nominal concentration of 1.5 mg/mL Recent move to K2EDTA from K3EDTA for hematology to reduce affect on RBC parameters. - The requisition form had the patient's name, and ordering physician. endstream Requesting physician's complete name, signature and stamp. 2022 I. Template SPECIMEN The recovery of anaerobes is compromised if the transport time exceeds 3hr. - The research report includes specific segments by region (country), by company, by Type and by Application. Methodologies and Regulations in Specimen Collection and Management - . 5. 1-2 gm quantity is sufficient. BD offers Vacutainer blood sample collection tubes in both glass and plastic. (ii) For special cases (examination of endocrines and viral infection-like PPR) tissues are to be collected in Bouin's fluid. 363 0 obj <> endobj ABSTRACT The 316-hectare (782-acre) Arroyo Hondo Preserve is located along the Gaviota Coast, 48 kilometers (30 miles) west of Santa Barbara, California. The integrity of the sample must be preserved and requirements for collection and handling must be followed. Specimen collection and preservation Oct. 17, 2012 65 likes 48,044 views Health & Medicine Its about various details of collection of specimen, techniques, presevation and processing. Our new CrystalGraphics Chart and Diagram Slides for PowerPoint is a collection of over 1000 impressively designed data-driven chart and editable diagram s guaranteed to impress any audience. Collect 5-10 ml of venous blood in a dry sterile, screw-cup glass tube or bottle. A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. A, Acute meningitis is a medical emergency - it can, Acute meningitis is commonly caused by only a few. Errors Anticoagulant cross contamination Citrate : If drawing a blue top tube for coagulation tests, (other than PT and PTT), a red tube (2-3 cc) must be drawn first to avoid contamination from tissue thromboplastin, which can yield false coagulation Results. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. MGM medical college ,mumbai Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. 379 0 obj <>/Filter/FlateDecode/ID[<0418542909B6434FA5DC5F39696DAE8B>]/Index[363 31]/Info 362 0 R/Length 89/Prev 695974/Root 364 0 R/Size 394/Type/XRef/W[1 3 1]>>stream Specimen collection 130319145806-phpapp02, Medical Microbiology Laboratory (sample collection), Sample collection and safety procedure in laboratory, Collection, transport & storage of clinical specimens, specimen collection&handling,Lusubilo2012, Collection and Handling of Specimens for Laboratory Diagnosis, Specimen collection and transport Dr.Ashna Ajimsha, Collection of biological specimens for microbiology tests, Collection and transport of clinical samples, Collection, Preservation and Dispatch of Clinical Samples, U. P. Veterinary University (DUVASU), Mathura, Selection ,collection, transportation and processing of mycology specimen, Laboratory diagnosis of_infectious_diseases. The isolation of viruses from the rectal swabs,s however, is less satisfactory than from feces. Refrigerate immediately at 4C, and then transport in a cold box. Winner of the Standing Ovation Award for Best PowerPoint Templates from Presentations Magazine. Role of specimen collection in infectious disease by Dr. T. V. Rao MD . The SlideShare family just got bigger. EDTA induced psuedothrombocytopenia caused by in vitro antibody mediated platelet clumping and platelet satellitism that results from changes in membrane structure that occur when calcium is removed by EDTA. Requisition form had the patient and the puncture site sterile mucus extractor podcasts and more box... Safe transport and biosafety, by company, by company, by company, by Type and Application! 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